Discovery HS F5 HPLC Columns

SIGMA ALDRICH
Unique Retention and Selectivity Enables Better Separations
Discovery® HS F5 Exhibits "U-Shape" Retention Profile

Under certain mobile phase conditions and with certain analytes, certain polar phases, like Discovery® HS F5, can exhibit both reversed-phase and normal-phase behavior. At low percent organic, retention decreases with increasing percent organic following reversed-phase behavior. However, at higher percent organic, retention increases with increasing percent organic following normal-phase behavior. The result is a "U-shape" retention profile for these compounds. If your compounds exhibit this U-shape profile, use it to your advantage to:
  • Improve LC-MS detection by using higher % organic mobile phase
  • Use mobile phase concentration to alter selectivity at high % organic

HS F5 bonded fluoro (pentafluorophenyl) columns provide separations that are different from C18 columns. Unique selectivity plus the ability to transfer a C18 method make this an ideal complimentary reversed-phase for screening.

Key Applications

Small, water soluble molecules and peptides, polar compounds, basic compounds, positional isomers

Notes

  • Compared to a phenyl phase: Although aromatic in nature, the pentafluorophenylpropyl (F5) phase does not resemble a phenyl phase in retention or selectivity. The F5 is a strong Lewis acid due to the electron withdrawing effects of five fluorine groups; the F5 ring is electron deficient whereas the phenyl ring is electron rich.
Discovery HS F5 HPLC Columns

USP Code

L43

Loading

12% Carbon

Particle platform

Type B Silica gel, Spherical

Phase

Pentafluorophenylpropyl

Surface coverage

4 μmol/m2

Metals

< 10 ppm

Surface area

300 m2 /g

Endcapped

Yes

Pore Size

120 Å

Particle size

3, 5, and 10 μm

Operating pH range

2 – 8



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