Ficoll PM 400

A highly stable sucrose polymer with an average molecular weight of 400 000, suitable for isolating eukaryotic cells and organelles with minimal damage.
  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
  • Better osmotic properties than sucrose.
  • Preserves functional and morphological integrity.
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
  • Does not penetrate biological membranes.
  • Serves as the raw material for preparation of Ficoll-Paque gradients.
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.


Cell isolation

Density Max.

1.2 g/ml

Endotoxin Activity Max.

0.12 EU/mg

Estimated shelf life from manufacture date

5 years

Storage conditions



Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Physical State


Physical Form

Spray-dried powder

Molecular Weight (Mr) Max.

~ 5 × 10~ 5

Molecular Weight (Mr) Min.

~ 3 × 105

Stokes Radius

~ 10 nm



Specific Rotation (alpha)20


Relative Viscosity

5 ηr at 20°C: 10% (w/v) 20 ηr at 20°C: 20% (w/v) 60 ηr at 20°C : 30% (w/v 180 ηr at 20°C : 40% (w/v) 600 ηr at 20°C : 50% (w/v)

Intrinsic Viscosity

~ 17 ml/g

Certificate of Analysis