Ascentis C8 HPLC Columns

SIGMA ALDRICH

One of The Most Hydrophobic C8 Phases Available

Leveraging the improvements to silica and bonded phase properties that made Ascentis C18 so useful, its shorter alkyl chain cousin, Ascentis C8, is also suitable for routine HPLC and LC-MS.

Ascentis C8 is suitable for any method that specifies a C8-type column. Although C8 columns often show similar selectivity to C18 columns, shorter alkyl chains sometimes show different selectivity toward polar compounds because they can solvate differently with the mobile phase and interact differently due to the size and shape of certain molecules. Also, C8 reagents are smaller than C18 reagents and have improved primary phase coverage, thereby requiring less endcapping. Ascentis C8 has excellent peak shape and very high phase stability.

Among commercially available C8 columns, Ascentis C8 has the highest degree of hydrophobic retention. This permits the use of higher percentages of organic modifier, a benefit to LC-MS users.

Ascentis C8 HPLC Columns

USP Code:

L7

Bonded Phase Description:

Octyl

Endcapped:

Yes

Particle composition:

Type B silica gel

Particle Purity:

< 5 ppm metals

Particle Shape:

Spherical

Particle Size:

3, 5 and 10 µm

Pore Size:

100 Å

Surface Area:

450 m2/g

Carbon Load:

15 %

pH Range (recommended):

2 to 8

Extended pH range*:

1.5 to 10



Ordering Information for Ascentis C8 HPLC columns

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