High Surface Area and High Surface Deactivation Combine to Give Ascentis Silica Exceptional Performance as a Normal Phase, HILIC and Preparative HPLC Material
Besides being the underlying support for all Ascentis phases, Ascentis Silica has applications in its own right. Silica is widely used to separate positional isomers in normal phase mode, and polar compounds in HILIC (aqueous normal phase modes). Silica is also used in organic synthesis to purify reaction mixtures. In each case, a high purity, controlled and uniform surface is necessary to impart the desirable chromatographic performance.
The classic use of silica columns is for normal phase HPLC. The rigid structure of the silica surface, as opposed to the flexible nature of bonded phases, allows it to distinguish between molecules with different footprints that may have the same hydrophobicity. Normal phase is also widely used in preparative chromatography because the mobile phase is more easily removed by evaporation than the water-containing reversed-phase mobile phases.